Results

• Composite Part Was Mapped. The Composite Part has its own page.
• The Measurement was Excellent. The Measurement has its own page.

• We abandoned many low-priority goals but succeeded at our highest-priority goal: the microbial manufacture of three very useful protein products.
• Along the way, we produced a new biological part and executed some good measurements.
• Incidentally, we achieved some success with clinostat design and human practices.

This is a list of things that went wrong:

• We did not keep track of early ChatGPT brainstorms for Nico's ESM project.
• We did not use our physical wet lab time efficiently.
• We did not optimize our repolymerization.

The four charts below show our mechanistic fit (solid lines) against the measured HPLC data (dots) for BHET, MHET, and TPA release, one chart per ICCG condition (dockerin fusion present or absent, crossed with scaffold present or absent). See the Model page for the interactive version and the full governing equations.

All four charts share the same axes, Time (hours) against Concentration (µM), and the same legend: BHET (model) in orange, MHET (model) in blue, TPA (model) in green, and measured points (HPLC) as dots on each curve.

Dock+, Scaf+

Dock+, Scaf+: BHET, MHET, and TPA vs. time, model fit and measured HPLC points. Static line chart for condition Dock+, Scaf+. X axis is time in hours from 0 to 96. Y axis is concentration in micromolar from 0 to 3000. Three model curves are shown: BHET in orange, MHET in blue, and TPA in green, each overlaid with four measured HPLC data points at 24, 48, 72, and 96 hours in the matching color.

Dock+, Scaf−

Dock+, Scaf−: BHET, MHET, and TPA vs. time, model fit and measured HPLC points. Static line chart for condition Dock+, Scaf−. X axis is time in hours from 0 to 96. Y axis is concentration in micromolar from 0 to 3000. Three model curves are shown: BHET in orange, MHET in blue, and TPA in green, each overlaid with four measured HPLC data points at 24, 48, 72, and 96 hours in the matching color.

Dock−, Scaf+

Dock−, Scaf+: BHET, MHET, and TPA vs. time, model fit and measured HPLC points. Static line chart for condition Dock−, Scaf+. X axis is time in hours from 0 to 96. Y axis is concentration in micromolar from 0 to 3000. Three model curves are shown: BHET in orange, MHET in blue, and TPA in green, each overlaid with four measured HPLC data points at 24, 48, 72, and 96 hours in the matching color.

Dock−, Scaf−

Dock−, Scaf−: BHET, MHET, and TPA vs. time, model fit and measured HPLC points. Static line chart for condition Dock−, Scaf−. X axis is time in hours from 0 to 96. Y axis is concentration in micromolar from 0 to 3000. Three model curves are shown: BHET in orange, MHET in blue, and TPA in green, each overlaid with four measured HPLC data points at 24, 48, 72, and 96 hours in the matching color.

Measured data

Concentrations in µM, from HPLC quantification of the APET film degradation assay: 5 mL reaction, 100 mU·mL⁻¹ ICCG, 6×6×0.5 mm APET film chip. “Dock+” = dockerin-fused ICCG–DoT; “Dock−” = unfused ICCG. “Scaf+” = co-incubated with 1 µg·mL⁻¹ ScafGVT.

See the Model page for the full governing equations and fitted rate constants behind these curves.

• We have the resources to engineer one specific microbial factory, but the products of our factory have to be applied in a PETosome, and we cannot make our own PETosome.
• Also, for use in space travel, a crucial step is to re-assemble the components, and our technology is compatible with Candida-based reassembly, but we do not produce our own re-assembly factory.

• We are likely to extend the technology to create microbial factories to other types of factories.
• We lack an acceptable means of storing old biological samples and re-using them or building on them.
• We lack expertise in many of the specialized organisms that we want to use (e.g. Candida variants).
• Our biggest challenge will be choosing a topic that will allow us to emphasize our engineering skills.